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Free Medis2/17/2024 ![]() Chemically defined conditions for human iPSC derivation and culture. Insulin-like growth factors and insulin control a multifunctional signalling network of significant importance in cancer. Massoner, P., Ladurner-Rennau, M., Eder, I. A transcriptomic study of myogenic differentiation under the overexpression of PPARĪ³ by RNA-Seq. A restriction point for control of normal animal cell proliferation. The mammalian myosin heavy chain gene family. Pax7 is required for the specification of myogenic satellite cells. Molecular cloning of caveolin-3, a novel member of the caveolin gene family expressed predominantly in muscle. Neural cell adhesion molecule (NCAM) marks adult myogenic cells committed to differentiation. Inactivation of Rho/ROCK signaling is crucial for the nuclear accumulation of FKHR and myoblast fusion. Identification of genes differentially expressed in myogenin knock-down bovine muscle satellite cells during differentiation through RNA sequencing analysis. Transcriptomic dissection of myogenic differentiation signature in caprine by RNA-Seq. Characterization of transcriptional complexity during longissimus muscle development in bovines using high-throughput sequencing. Gene expression during normal and FSHD myogenesis. Myomaker: a membrane activator of myoblast fusion and muscle formation. Making muscle: skeletal myogenesis in vivo and in vitro. Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing. Differentiation of pluripotent stem cells to muscle fiber to model Duchenne muscular dystrophy. In vitro differentiation of functional human skeletal myotubes in a defined system. Developing a novel serum-free cell culture model of skeletal muscle differentiation by systematically studying the role of different growth factors in myotube formation. Textured soy protein scaffolds enable the generation of three-dimensional bovine skeletal muscle tissue for cell-based meat. Tissue-engineered skeletal muscle organoids for reversible gene therapy. Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. Regeneration of single skeletal muscle fibers in vitro. Scientific, sustainability and regulatory challenges of cultured meat. The potential of future foods for sustainable and healthy diets. Moreover, the serum-free differentiation media supported the fabrication of three-dimensional bioartificial muscle constructs, demonstrating its suitability for cultured beef production. Serum-free myogenic differentiation was of similar extent to that induced by serum starvation, as evaluated by transcriptome analysis, protein expression and the presence of a functional contractile apparatus. Supplementation of ligands to these receptors enabled the formulation of a chemically defined media that induced differentiation in the absence of serum starvation and/or transgene expression. We characterized canonical myogenic gene expression, and identified surface receptors upregulated during the early phase of differentiation, including IGF1R, TFRC and LPAR1. Here we used RNA sequencing to investigate the transcriptomic remodelling of bovine satellite cells during myogenic differentiation induced by serum starvation. Current protocols induce myogenic differentiation in vitro through serum starvation, that is, an abrupt reduction in serum concentration. ![]() Cultured meat production requires the robust differentiation of satellite cells into mature muscle fibres without the use of animal-derived components.
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